Device

Part:BBa_K533009:Design

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)

Tar receptor weak expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1074
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1074
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1074
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2514
    Illegal BsaI site found at 3582
    Illegal SapI.rc site found at 765


Design Notes

Tar lacks its own promoter, which leads to lower expression level and more sensitive chemotaxis.

Source

Kind gift from Dr. Goulian.

References